Features of the inflammatory response of monocytes in patients with premature coronary artery disease
DOI:
https://doi.org/10.34687/2219-8202.JAD.2024.03.0006Keywords:
premature coronary artery disease, monocytes, inflammatory cytokines, immune toleranceAbstract
Objective. Impaired monocyte/macrophage inflammatory response is an important mechanism in the pathogenesis of atherosclerosis The aim of the study was to investigate the secretion of inflammatory cytokines by cultured monocytes/macrophages from patients with premature coronary artery disease (CAD).
Materials and methods. The study included 73 people: 38 patients with CAD, 35 patients without CAD, matched by gender and age. A primary culture of CD14+ monocytes was obtained by immunomagnetic separation. To stimulate the inflammatory response, LPS was added to the cell culture for 24 hours on days 1 and 6. Basal and LPS-stimulated secretion of the cytokines TNF-α, IL-1β, IL-6, IL-8 and MCP-1 was assessed by ELISA on days 2 and 7 of cultivation. Logistic regression analysis was performed to assess the role of the inflammatory response of monocytes along with traditional cardiovascular risk factors in the development of CAD.
Results. The levels of basal secretion of TNF-α, IL-1, IL-6, MCP-1 were higher in patients with CAD compared to participants in the control group. The level of re-stimulated secretion of TNF-α and levels of LPS-stimulated and re-stimulated secretion of IL-1 on the second and sixth days also were higher in the group with CAD. Levels of stimulated IL-6 secretion didn’t differ between the groups. Neither basal nor stimulated secretion of IL-8 differed between the CAD and control groups. LPS-stimulated MCP-1 secretion on the second day did not differ in CAD and control group. Restimulated MCP-1 secretion was higher in the CAD group. The results of logistic regression analysis showed that the secretion of IL-1β and IL-6 is independent predictor of the premature CAD, along with smoking, body mass index and serum HDL cholesterol levels.
Conclusion. Thus, the study revealed pro-inflammatory activation of monocytes/macrophages in patients with premature CAD, which consists of an increase in the basal and induced secretion of inflammatory cytokines TNF-α, IL-1β, MCP-1 and, to a lesser extent, IL-6.
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