Расчетный метод определения уровня холестерина липопротеинов низкой плотности на основании современной парадигмы метаболизма липидов

P. S. Sadovnikov; A. Yu. Olkhovik; V. S. Gurevich

doi:10.34687/2219-8202.JAD.2022.03.0003

Calculation method for the low-density lipoprotein cholesterol levels on the basis of the lipid metabolism modern paradigm

Authors

P. S. Sadovnikov
A. Yu. Olkhovik
V. S. Gurevich

DOI:

https://doi.org/10.34687/2219-8202.JAD.2022.03.0003

Abstract

Aim. Using the Friedwald formula to determine the level of low-density lipoprotein cholesterol (LDL-C) in the blood leads to underestimated results compared to real values. The purpose of this work was to improve the calculation formula for LDL-C.
Materials and methods. 750,000 results of complete individual lipidogramms were analyzed. Data processing was performed using the Python programming language version 3.9.2 using software libraries: pandas (1.1.3), numpy (1.19.2), seaborn (0.11.0), matplotlib (3.3.2), statsmodels (0.12.0), sklearn (0.23.2). The accuracy of the model in the determination of the analyte was evaluated using the Allowable Total Error (TEa) = 11.9%.
Results. According to the direct measurement of the level of total cholesterol, HDL-C, LDL-C and TG, a graph of the dependence of the level of VLDL-C on the content of triglycerides in all fractions of lipoproteins was constructed. The coefficients of the intermediate equations were obtained from the linear regression equation, fitted by the least squares method using the statsmodels library (0.12.0) The final calculation formula was the following: LDL-C = nonHDL-C – (TG / 3 – 0.14). The accuracy of this equation in comparison with the direct determination of LDL-C in terms of TEa was 96.12% on a test sample of 150,000 lipidograms, which is significantly higher than when calculating using the Friedwald formula and alternative methods of S. Martin and M. Sampson: 62.29%, 72.96%, 71.62% respectively.
Conclusion. The new calculation formula for determining LDL-C proved to be a suitable tool, easy to use in any triglyceride levels. The use of this formula makes it possible to adequately compare the results of the calculated and direct assessments of LDL-C.

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References

Kukharchuk VV, Ezhov MV, Sergienko IV, Arabidze GG, Bubnova MG, Balakhonova TV, et al. Diagnosis

and correction of lipid metabolism disorders for the prevention and treatment of atherosclerosis. Russian

recommendations, VII revision. Atherosclerosis and Dyslipidemia. 2020;1:7-40. In Russian. (Кухарчук В.В., Ежов М.В.,

Сергиенко И.В., Арабидзе Г.Г., Бубнова М.Г., Балахонова Т.В. и др. Диагностика и коррекция нарушений липидного обмена с целью профилактики и лечения атеросклероза. Российские рекомендации, VII пересмотр. Атеросклероз и дислипидемии. 2020;1(38)7-40).

Friedewald WT, Levy RI, Fredrickson DS. Estimation of the concentration of low-density lipoprotein cholesterol in

plasma, without use of the preparative ultracentrifuge. Clin Chem. 1972;18(6):499-502.

Parvin M, Saiedullah M, Khan M, Rahman M. Validation of the Modified Friedewald’s Formula to Calculate Lowdensity Lipoprotein Cholesterol in Bangladeshi Population. J Bangladesh Coll Physicians Surg. 2012;30(3):141-144.

doi: 10.3329/jbcps.v30i3.12463.

Mendes de Cordova CM, de Santa Helena ET, Galgowski C, Figueira VH, Setter GB, Markus MRP, et al. Evaluation

of a new equation for LDL-c estimation and prediction of death by cardiovascular related events in a German

population-based study cohort. Scand J Clin Lab Invest. 2018;78(3):187-196. doi: 10.1080/00365513.2018.1432070.

Krishnaveni P. Assessing the Validity of Friedewald’s Formula and Anandraja’s Formula For Serum LDL-Cholesterol

Calculation. J Clin Diagn Res. 2015;9(12):BC01-4. doi: 10.7860/JCDR/2015/16850.6870.

Okada M, Matsui H, Ito Y, Fujiwara A, Inano K. Low-density lipoprotein cholesterol can be chemically measured: a

new superior method. J Lab Clin Med 1998;132:195-201. doi: 10.1016/s0022-2143(98)90168-8.

Bansal E, Kaur N. Does Friedewald Formula Underestimate the Risk of Ischemic Heart Disease? Indian J Clin

Biochem. 2014 Oct;29(4):496-500. doi: 10.1007/s12291-013-0392-2.

Martin SS, Blaha MJ, Elshazly MB, Toth PP, Kwiterovich PO, Blumenthal RS, et al. Comparison of a Novel Method

vs the Friedewald Equation for Estimating Low-Density Lipoprotein Cholesterol Levels From the Standard Lipid

Profile. JAMA. 2013;310(19):2061-2068 doi: 10.1001/jama.2013.280532.

Grundy SM, Stone NJ, Bailey AL, Beam C, Birtcher KK, Blumenthal RS, et al. 2018 AHA/ACC/AACVPR/AAPA/

ABC/ACPM/ADA/AGS/APhA/ASPC/NLA/PCNA Guideline on the Management of Blood Cholesterol: A Report of the

American College of Cardiology/American Heart Association Task Force on Clinical Practice Guidelines. Circulation.

;139(25)2805-2808. doi: 10.1161/CIR.0000000000000625.

Sampson M, Ling C, Sun Q, Harb R, Ashmaig M, Warnick R, et al. A New Equation for Calculation of Low-Density

Lipoprotein Cholesterol in Patients With Normolipidemia and/or Hypertriglyceridemia. JAMA Cardiol. 2020;5(5):540-

doi: 10.1001/jamacardio.2020.0013.

Morita SY. Metabolism and Modification of Apolipoprotein B-Containing Lipoproteins Involved in Dyslipidemia and

Atherosclerosis. Biol Pharm Bull. 2016;39(1):1-24. doi: 10.1248/bpb.b15-00716.

Kumari A, Kristensen KK, Ploug M, Winther AML. The Importance of Lipoprotein Lipase Regulation in

Atherosclerosis. Biomedicines. 2021;9(7):782. doi: 10.3390/biomedicines9070782.

https://www.westgard.com/vp-helix-laboratories.htm.

Elevitch FR. Proceedings of the 1976 Aspen Conference on Analytical Goals in Clinical Chemistry.

https://www.westgard.com/biodatabase1.htm.

Ricуs C, Alvarez V, Cava F, Garcнa-Lario JV, Hernбndez A, Jimйnez CV et al. Current databases on biological

variation: pros, cons and progress. Scand J Clin Lab Invest. 1999;59(7):491-500. doi: 10.1080/00365519950185229.

Oldoni F, Baldassarre D, Castelnuovo S, Ossoli A, Amato M, van Capelleveen J, et al. Complete and partial lecithin:

cholesterol acyltransferase deficiency is differentially associated with atherosclerosis. Circulation. 2018;138:1000-1007.

doi: 10.1161/circulationaha.118.034706.

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2022-10-04

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Sadovnikov P. S., Olkhovik A. Y., Gurevich V. S. Calculation method for the low-density lipoprotein cholesterol levels on the basis of the lipid metabolism modern paradigm // The Journal of Atherosclerosis and Dyslipidemias. 2022. VOL. № 3 (48). PP. 21–28.

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Calculation method for the low-density lipoprotein cholesterol levels on the basis of the lipid metabolism modern paradigm

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