Toxicity of calcium phosphate bions for aortic adventitia in rats

Abstract

Aim: To investigate whether calcium phosphate bions (CPB) induce adventitial inflammation.
Material and Methods. Toxicity of spherical calcium phosphate bions (CPBS), needle-shaped calcium phosphate bions (CPBN), and magnesium phosphate bions (MPB) for aortic intima of Wistar rats was assessed by intravenous administration immediately upon the angioplasty. After 5 weeks, rats were sacrificed, and injured aortic segments were then examined utilizing hematoxylin and eosin staining.
Results. In contrast to CPBS and CPBN, MPB did not provoke intimal hyperplasia. Neointima formation was induced by both mechanical injury and endothelial toxicity of CPB. This also triggered adventitial inflammation as demonstrated by an increased count of lymphoid-like follicles.
Conclusion. Intravenous administration of CPB after the balloon injury caused intimal hyperplasia of rat abdominal aortas and stimulated the formation of adventitial lymphoid-like follicles. This points on the association between CPB endothelial toxicity and adventitial inflammation.