The efficacy and mechanisms of action of the peptide Lys-Glu-Trp model hyperlipidemia caused by administration of Tween-80

Abstract

Aim. The aim of the study was to determine the dose-dependent effects of peptide Lys-Glu-Trp on the model of experimental hyperlipidemia caused by the introduction of Tween-80. Material and methods. Fort he formation of the pathology on the 6th day of the study the animals were injected intraperitoneally with Tween-80 at a dose of 200 mg I 100 g. The test drugs were administered intraperitoneally for 6 days before induction of disease. The study included groups: intact (without treatment, without pathology), control (pathology, placebo 0.9% sodium chloride), study groups treated with peptide lysyl-glutamyl-tryptophan (Lys-Glu-Trp) at doses 0.2 and 2 mg/kg and the group treated with the reference drug atorvastatin dose of 5.6 mg/kg. Each group consist of 10 male Wistar rats. On the 7th day of the experiment the blood was collected. The blood plasma was investigated on the parameters: total cholesterol (TC); high density lipoproteins; low density lipoproteins; triglycerides; bloodglucose; bilirubin; lecithin-cholesterol acyltransferase (LCAT). Results. The study found marked lipid-lowering effect of the peptide Lys-Glu-Trp, realised mainly due to lower levels of atherogenic LDL. Investigated tripeptide efficiency test in small doses, and no differences in its efficacy at doses of 0.2 and 2 mg/kg can be the basis for further studies of its efficacy and safety as a means for the treatment and prevention of atherosclerosis. Conclusions. The mechanism of action of the test peptide Tys-Glu-Trp may be associated with the influence on the system proteins that regulate metabolism and transportation of TC, in particular the activity of LCAT. As a result, this study established the ability to reduce the severity of the fall of the tripeptide concentration of LCAT in  the background experimental hyperlipidemia by 49-55%.