Locus 9р21.3 – genetic predictor of coronary atherosclerosis severity

Abstract

The purpose of this study was to investigate association between 9p21.3 locus single nucleotide polymorphisms (SNP) and severity of coronary atheromatous burden. A total of 255 Caucasians (211 male, 44 female) with myocardial infarction age younger 65 old (mean 52,56±7,98 years) were recruited from 01.01.2009 to 30.06.2010. The study was approved by Ethic Committee of the KrasGMU. All participants were included in the study after written informed consent form. Genome DNA was extracted from leukocytes of venous blood using the phenol-chloroform extraction method. Two SNPs rs10757278 and rs1333049 (locus 9p21.3) were tested using real-time polymerase chain reaction (PCR) according to protocol (probes TaqMan, Applied Biosystems, 7900HT). The coronary angiograms were reviewed by independent angiographers who were blinded to the results of the genotype. The total number of lesions, Gensini scores and SYNTAX score were derived. For the first time in Russian population, genotype CC rs1333049 and genotype GG rs10757278 locus 9р21.3 demonstrated a direct strong association with severity of coronary atheromatous burden