Взаимосвязь провоспалительной активации моноцитов с факторами риска атеросклероза при сахарном диабете 2 типа

T. V. Kirichenko; L. A. Bochkareva; L. V. Nedosugova; Yu. V. Markina; I. A. Kuzina; T. V. Tolstik; A. I. Bogatyreva; A. M. Markin

doi:10.34687/2219-8202.JAD.2024.01.0006

Authors

T. V. Kirichenko Research Institute of Human Morphology named after Academician A.P. Avtsyn «Russian State Scientific Center for Surgery named after Academician B.V. Petrovsky»
L. A. Bochkareva First Moscow State Medical University named after Sechenov
L. V. Nedosugova First Moscow State Medical University named after Sechenov
Yu. V. Markina Research Institute of Human Morphology named after Academician A.P. Avtsyn «Russian State Scientific Center for Surgery named after Academician B.V. Petrovsky»
I. A. Kuzina First Moscow State Medical University named after Sechenov
T. V. Tolstik Research Institute of Human Morphology named after Academician A.P. Avtsyn «Russian State Scientific Center for Surgery named after Academician B.V. Petrovsky»
A. I. Bogatyreva Research Institute of Human Morphology named after Academician A.P. Avtsyn «Russian State Scientific Center for Surgery named after Academician B.V. Petrovsky»
A. M. Markin Research Institute of Human Morphology named after Academician A.P. Avtsyn «Russian State Scientific Center for Surgery named after Academician B.V. Petrovsky»

DOI:

https://doi.org/10.34687/2219-8202.JAD.2024.01.0006

Keywords:

diabetes mellitus, atherosclerosis, monocytes, proinflammatory cytokines

Abstract

Objective: Chronic inflammation plays an important role in the pathogenesis of type 2 diabetes, leading to active progression of atherosclerosis, which significantly increases cardiovascular mortality in patients with diabetes. The aim of the study was to assess the pro-inflammatory monocyte activation in type 2 diabetes and to determine its association with clinical characteristics of patients with type 2 diabetes, including cardiovascular risk factors and indicators of atherosclerosis.
Materials and methods: The study included 50 patients with newly diagnosed type 2 diabetes and 40 participants of the control group. The study participants were assessed for cardiovascular risk factors such as BMI, blood lipid profile, and indicators of carotid atherosclerosis. To characterize the proinflammatory activation of monocytes, basal and LPS-stimulated secretion of the
cytokines TNF-α, IL-6, IL-8 and MCP-1 was assessed in the primary culture of monocytes of study participants.

Results: Cardiovascular risk factors were significantly increased in the group of patients with type 2 diabetes, in particular, high BMI, high levels of triglycerides and LDL and reduced levels of HDL, increased cIMT of the carotid arteries and significantly higher degree of atherosclerotic carotid stenosis. The level of basal secretion of TNF-α, IL-6, IL-8 and MCP-1 was significantly higher in the group of patients with type 2 diabetes compared to participants in the control group. The levels of LPS-stimulated secretion were also higher in patients with type 2 diabetes, but only the chemokines IL-8 and MCP-1 showed significant differences between the groups. As a result of the correlation analysis, an association between IL-6 secretion and the levels of total cholesterol and triglycerides in the blood serum, an association between MCP-1 secretion and the level of triglycerides, and an association between TNF-α and IL-8 secretion and atherosclerotic stenosis in the carotid artery were revealed.
Conclusion: Thus, the study revealed pro-inflammatory activation of monocytes in patients with type 2 diabetes compared with participants of the control group, associated with atherosclerosis of the carotid arteries and blood lipid profile indicators, which is of interest for the development of new diagnostic algorithms and therapeutic strategies for diabetes 2 types.

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