The influence of lipid transport system genes polymorphism combined carriage on lipid parameters in patients with coronary atherosclerosis

Abstract

Objective. To study features of lipid metabolism according to the combined carriage of «ε4» allele of the ε2/ε3/ε4 apolipoprotein E (АРО E) gen polymorphism and of «S2» allele of the SstI apolipoprotein CIII (АРО СIII) gen polymorphism in patients with unstable angina (UA) and coronary atherosclerosis.
Material and methods. 141 patients with UA and coronary atherosclerosis and 50 healthy volunteers were observed. Lipids and apolipoproteines were defined on biochemical autoanalyzer «Daytоna» (RANDOX, Ireland). ε2/ε3/ε4 polymorphism of АРО E gene and SstI polymorphism of АРО СIII gene definition was performed by the restriction fragment length polymorphism polymerase chain reaction (RFLP-PCR) method. Coronary angiography was performed using Allura CV-20 (Philips, Netherlands).
Results. While analysing the frequency distribution of «ε4» allele of АРО E gene among UA patients, «ε4» allele carriers were observed more often – 87 (61.7%), in comparison with healthy persons – 54 (38.3%) (OR 11.82; 95% CI 4.7–29.6; χ2=34.535, p<0.001). «S2» allele carriers also prevailed – 51 (27.6%), in comparison with healthy volunteers – 9 (18%) (OR 2.58; 95% CI 1.161–5.740, χ ² =4.844, p<0.05). In this connection all patients have been divided into 2 groups: 37 patients (26.2%, I group) with a combination of «damaging» «ε4» and «S2» alleles against the others – 104 patients (73.8%, II group). In I group of patients total cholesterol (TC) (238.0±54.3) and low density cholesterol (LDL-C) levels (154.7±51.5) were higher, than in the II group (220.6±39.8 and 154.7±51.5, p<0.05), accordingly. Simultaneously, in I group of patients there was a significantly lower value of high density cholesterol (HDL-C) level (34.1±6.3) in comparison with II group (36.8±6.8, p<0.05). Аpolipoprotein В/apolipoprotein АI ratio at I group (0.9±0.3) was above (p<0.05), concerning II group (0.8±0.2).
Conclusions. Carriage of «ε4» allele of the ε2/ε3/ε4 APO E gen polymorphism and «S2» allele of the SstI APO CIII gen polymorphism is the promoting factor in development of a coronary atherosclerosis and atherogenic dislipidemia among Uzbek patients.