The polymorphisms of apolipoprotein E and lipoprotein lipase genes in evaluating the effectiveness of the hypocaloric standard diet in obese patients

Abstract

Aim. Aim of the study is to evaluate the effectiveness of the hypocaloric standard diet therapy for various polymorphic variations of lipoprotein lipase (LPL) and apolipoprotein E (ApoE) genes in obese patients.
Materials and methods. The study involved 110 obese patients with different allelic variants of ApoE and LPL genes. Lipid metabolism evaluation and analysis of ε2, ε3, ε4 polymorphic alleles of ApoE gene and Ser447Ter of LPL gene using polymerase chain reaction are performed. The concentrations of total cholesterol (ТС), triglycerides (TG), low density lipoproteins (LDL), high density lipoproteins (HDL) are measured by turbidimetry and spectrophotometry by using an automatic analyzer ConeLab60i (Finland).
Results. We concluded that the effectiveness of dietary intervention in lipid and carbohydrate metabolism disturbances in obese patients significantly higher in ε3/ε3 genotype of ApoE gene (19% reduced TC, 18% – TG, 17% – LDL, 20% – HDL, 7% – glucose), ε2/ε3 genotype of ApoE gene (20% reduced TC, 23% – TG, 15% – HDL) and in Ser447Ser genotype of LPL gene (17% reduced TC, 21% – TG, 14% – HDL, 16% – of LDL, 8% – glucose). 

Conclusion. Thus, the definition of ApoE and LPL gene polymorphisms can be used as a prognostic test in patients with an overweight and for developing of a personalized diet therapy in obese patients.