Plasmalogic phospholipids in myocardial hypoxia and experimental hypoxia

Abstract

Target. To assess the change in the content of plasmalogic phospholipids in the composition of erythrocytes during experimental hypoxia and myocardial hypoxia. Materials and methods. The object of the study was blood samples from patients with coronary artery disease (CHD), exertional angina and atherosclerosis of the coronary arteries. Served as control blood samples of practically healthy volunteers. In addition, isolated whole blood samples from practically healthy volunteers were analyzed after incubation at 37 ° C for 180 minutes. In this case, whole blood samples from the same volunteers, but not incubated, were used as control. Determination of the content of the analyzed substances was carried out using the method of capillary gas-liquid chromatography. Results. In the group of patients with ischemic heart disease, angina pectoris and atherosclerosis of the coronary arteries in comparison with healthy volunteers, an increase in the content of plasmalogic phospholipids in erythrocytes and a decrease in blood plasma pH were revealed. Conclusion. The data obtained in the course of the study indicate the need to correct metabolic acidosis as a cause of an increase in the content of plasmalogic phospholipids in the composition of erythrocyte membranes. Based on the data obtained, it is assumed that one of the factors increasing the content of plasmalogic phospholipids in the composition of cell membranes is a more significant increase in the activity of calcium-dependent phospholipase A, in comparison with the activity of calcium-independent plasmalogic phospholipase A₂ in comparison with the activity of calcium-independent plasmalogenous phospholipase A₂ against the background of hypoxia.